Holobiont Integration Physiological Analysis Pipeline

Montipora capitata and Pocillopora acuta fragments kept in the -80°C freezer.

Protocols:

1. Airbrushing
2. Total Protein
3. Chlorophyll-a
4. Symbiont counts using flow cytometry
5. Total Antioxidant Capacity
6. Ash-free dry weight
7. Total Lipid Content
8. Surface Area Wax-dipping

Once airbrushed, aliquot the following for each protocol:

• total protein: X μL
• chlorophyll-a: X μL
• total antioxidant capacity: X μL
• flow cytometry: X μL