Soft and Hard Homogenization DNA/RNA Extractions #83-90

20190723 E.S. DNA/RNA Extractions from Montipora capitata and Pocillopora acuta adult coral fragments from Holobiont Integration Hawaii 2018 project.

Soft and Hard homogenization, and DNA/RNA Extractions followed this protocol. General Zymo Duet DNA/RNA Extraction protocol found here.
Coral fragments were randomly chosen from different timepoint bags.

Timepoint	Species	Coral ID	Extraction ID	Homogenization
20180922	Montipora	1751	83	soft
20180922	Montipora	1751	84	hard
20181103	Montipora	1420	85	soft
20181103	Montipora	1420	86	hard
20181117	Montipora	1095	87	soft
20181117	Montipora	1095	88	hard
20181020	Montipora	1235	89	soft
20181020	Montipora	1235	90	hard

Fragment preparation start ~13:06 end 13:15
Soft/Hard homogenization end 13:45
Extractions end ~15:40

Qubit Results:

DNA (ng/μl)
Standard 1	179.71
Standard 2	20560.79
Coral ID	Extraction ID	Reading 1	Reading 2	Average
1751	83	34.6	34.2	34.4
1751	84	18.3	18.1	18.2
1420	85	34.8	34.4	34.6
1420	86	21	20.8	20.9
1095	87	13.8	13.8	13.8
1095	88	12.9	12.9	12.9
1235	89	34.8	34.6	34.7
1235	90	15.1	15.1	15.1

RNA (ng/μl)
Standard 1	407.62
Standard 2	10776.89
Coral ID	Extraction ID	Reading 1	Reading 2	Average
1751	83	17	16.8	16.9
1751	84	12.2	12.2	12.2
1420	85	37.2	37.2	37.2
1420	86	18.6	18.6	18.6
1095	87	18.6	15.8	17.2
1095	88	11.2	11	11.1
1235	89	23.2	23	23.1
1235	90	28.8	23.6	26.20

Gel Electrophoresis Results:

Extractions #: 83-90

TapeStation Results:

Coral Fragment ID	Extraction ID	RIN
1751	83	8.7
1751	84	8.6
1420	85	8.4
1420	86	8.5
1095	87	8.3
1095	88	8.4
1235	89	7.7
1235	90	7.9

Link to 20190723 report #3, Extractions #83-90