Soft and Hard Homogenization DNA/RNA Extractions 119-138

Soft and Hard Homogenization DNA/RNA Extractions #119-138

20190725 E.S. DNA/RNA Extractions from Montipora capitata and Pocillopora acuta adult coral fragments from Holobiont Integration Hawaii 2018 project.

Soft and Hard homogenization, and DNA/RNA Extractions followed this protocol. General Zymo Duet DNA/RNA Extraction protocol found here.
Coral fragments were randomly chosen from different timepoint bags.

Fragment preparation start ~9:20 end 9:50
Soft/Hard homogenization start 9:51 end 10:33
RNA DNA Extraction start 10:34 end ~13:30

Qubit used to check DNA and RNA quantity (ng/μl).
TapeStation used to check RNA quality.

DNA Standard 1: 215.21 ng/μl
DNA Standard 2: 24479.38 ng/μl
RNA Standard 1: 410.82 ng/μl
RNA Standard 2: 11192.82 ng/μl

Timepoint	Species	Coral ID	Extraction ID	Homogenization	DNA Reading 1	DNA Reading 2	Average DNA ng/μl	RNA Reading 1	RNA Reading 2	Average RNA ng/μl	RIN
20181020	Pocillopora	1765	119	soft	77.6	74.4	76	67.6	67	67.3	7.2
20181020	Pocillopora	1765	120	hard	51.4	49.6	50.5	37.6	37.2	37.4	6.7
20181117	Pocillopora	1536	121	soft	80	79	79.5	61.8	61.4	61.6	7.8
20181117	Pocillopora	1536	122	hard	58.4	58	58.2	49.4	48.8	49.1	7.6
20180922	Pocillopora	2993	123	soft	82.2	80.8	81.5	73.8	73.6	73.7	6.6
20180922	Pocillopora	2993	124	hard	48.6	48.2	48.4	49	48.8	48.9	6.3
20181117	Pocillopora	1238	125	soft	43.4	42.8	43.1	55.4	55	55.2	8.5
20181117	Pocillopora	1238	126	hard	27.6	27.2	27.4	31.8	31.6	31.7	8.1
20181020	Montipora	3753	127	soft	19.5	19.4	19.45	11.4	11.4	11.4	**
20181020	Montipora	3753	128	hard	12.3	12.1	12.2	10	10	10	**
20181117	Pocillopora	1559	129	soft	50.6	48.4	49.5	43.2	43	43.1	8.4
20181117	Pocillopora	1559	130	hard	35.6	35.2	35.4	31.6	31.2	31.4	7.8
20180922	Pocillopora	1060	131	soft	72.2	71.4	71.8	82	81.4	81.7	7.9
20180922	Pocillopora	1060	132	hard	44.6	44	44.3	50.2	50	50.1	7.7
20181117	Montipora	1478	133	soft	47.8	47.2	47.5	38.4	38.2	38.3	8
20181117	Montipora	1478	134	hard	36.2	35.8	36	24.2	24	24.1	8.1
20181103	Montipora	1331	135	soft	16	15.8	15.9	11.8	11.8	11.8	8.7
20181103	Montipora	1331	136	hard	10.9	10.7	10.8	**	**	**	**
20181103	Montipora	1306	137	soft	37.6	37	37.3	26.6	26.6	26.6	8.4
20181103	Montipora	1306	138	hard	31.8	31.6	31.7	13.8	13.8	13.8	8.5

Link to 20190725 TapeStation report, Extractions #119-138

Gel Electrophoresis Results:

20190725 Extractions #118-138

Extraction #121 was lighter in color before placing in well.
Extraction #137 did not settle well into the gel. Could be leftover ethanol in the sample.

Written on July 25, 2019