Qubit, Gel, Tape for re-dos
20191015 E.C.
Gel Electrophoresis, Qubit, and TapeStation re-dos for samples that did not read correctly the first time.
Qubit used to check DNA and RNA quantity (ng/μl).
20191014 Extractions
| Timepoint | Species | Coral ID | Extraction ID | Homogenization | DNA Reading 1 | DNA Reading 2 | Average DNA ng/μl | RNA Reading 1 | RNA Reading 2 | Average RNA ng/μl | RIN |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 20181215 | Pocillopora | 1147 | 515 | soft | 113 | 113 | 113 | 147 | 146 | 146.5 | 6.9 |
| 20181215 | Pocillopora | 1147 | 516 | hard | 32.4 | 32.2 | 32.3 | 55.2 | 55.4 | 55.3 | NA |
| 20190112 | Montipora | 1120 | 517 | soft | 25.2 | 25 | 25.1 | 10.6 | 10.8 | 10.7 | 9.1 |
| 20190112 | Montipora | 1120 | 518 | hard | 13.1 | 13.1 | 13.1 | ** | ** | ** | NA |
| Extraction ID | RNA Reading 1 | Reading 2 | Average RNA |
|---|---|---|---|
| 453 | 14.2 | 14.2 | 14.2 |
| 454 | 10.2 | 10.2 | 10.2 |
TapeStation used to check RNA quality.
Link to 20191015 TapeStation report, Extractions #25, 177, 179, 279, 477, 493, 515, 517
Written on October 15, 2019