DNA RNA Extraction for Pocillopora meandrina

DNA RNA Extraction using this protocol. Gel electrophoresis protocol here. Qubit protocol here. TapeStation for RNA protocol here.

Pocillopora meandrina fragments were vortexed for 1 minute. The same fragment was extracted twice to try to get the best RNA to use moving forward (PM-1 and PM-2).

Coral ID: Pocillopora meandrina bleached III Sep 2020 EN/PD that was shipped from Coral Resistance Lab in Hawaii.

Qubit and tapestation values

Qubit standards

DNA BR Standard 1: 188.36
DNA BR Standard 2: 20,073.85

RNA HS Standard 1: 43.71
RNA HS Standard 2: 715.98

PM-1

DNA Reading 1 = 81.6 ng/uL
DNA Reading 2 = 81.4 ng/uL
DNA Average = 81.5 ng/uL

RNA Reading 1 = 96.2 ng/uL
RNA Reading 2 = 96.0 ng/uL
RNA Average = 96.1 ng/uL

PM-2

DNA Reading 1 = 69.6 ng/uL
DNA Reading 2 = 69.0 ng/uL
DNA Average = 69.3 ng/uL

RNA Reading 1 = 102 ng/uL
RNA Reading 2 = 102 ng/uL
RNA Average = 102 ng/uL

Full tapestation report here

Gel electrophoresis image