E5 Timepoint 1, 2, 3, and 4 Instantaneous Calcification Sample Processing (Samples Collected in 2020)

Processing the E5 January, March, September, and November 2020 Instantaneous Calcification Samples

Original E5 processing post from D. Becker and March sample processing included here.

Putnam Lab Titration protocol here.

Kristen, Pierrick, and Emma will do the physical titrations according to the schedule above. Emma will do all steps in Titration Data Entry Process and analyze data after Kristen and Pierrick push to github.

The average # of runs per day was between 3-4 if the CRM and ph calibration were started first thing in the morning. Having multiple people to process was helpful to run a large # of runs per day (i.e. person 1 starts the run and person 2 switches the run over).

Daily log for notes about each step: google document link. Path is 201906_Post_Award > URI_Titrations > “E5 January, March, September, and November 2020 Instantaneous Calcification Samples Notes”.

Contents:

Issues/Questions to Address
Weekly Schedule
September 2020, 51 samples to complete
January 2020, 146 samples to complete
November 2020, 145 samples to complete
Equipment locations
Waste

Folders and file formats for this project

Path on desktop computer: Data > E5_Titrations > E5_2020_Calcification_Samples.

Each day make a new folder with the date YEAR-MONTH-DAY (i.e. 20220303).

Each CRM and sample run will have their own mass file instead of overriding the previous one.
Before running the R script, rename these files:

rename the LabX output file to match this format: 20220308_Run1_PutnamTitrations_PutnamLab (If CRM run, replace Run1 with “CRM”)
Mass files should reflect this format: Mass_CRM_20220308 (If a sample run, replace CRM with “Run #”)

After running the R script, rename these files:

rename the TA Output file to match this format: TA_Output_20220308_Run1_PutnamTitrations_PutnamLab (If CRM run, replace Run1 with “CRM”)

Notation for sample ID

Match this example sample ID format:

Colony-ID_DATE_RUN#
ACR-225_20200914_7
Initial1_20200915_10
BK-1_20200912_2
JUNK 1
CRM 1

CRM Information

March 3, 2022 - current:

Batch 180 Salinity: 33.623
Batch 180 TA value: 2224.47

Acid information

March 3, 2022 - current: Batch A22

Switched bottle: 20220324 (From Batch A22 to new Batch A22 bottle no. 54)

Salinity for 50 mL falcon tubes

Measure the salinity of the 50 mL falcon tubes prior to pouring the sample out into the titration cup. Thoroughly wipe the probe with a kim wipe in between samples and DI water so we don’t introduce any new liquid into the sample. Pour the entire sample into the titration cup and place the used 50 mL falcon in the same bin we are putting the processed brown bottle samples (in the walk in 4C cold room).

On the sample sheet, please mark down if the sample came from a 50 mL tube in the last column (mark yes or no).

Room temperature samples

Before starting the pH calibration, take samples out of the fridge so that they come down to room temperature by the time the run starts. We want to be measuring salinity and titration runs at room temperature.

Issues/Questions to Address

Open Issues

Issue: Salinity readings at CSUN vs URI (36 range vs 40 psu range..). Test batch effect. Calibrated with 50 mS/cm solution and then the probe was reading at ~55 when measuring the same solution post-calibration. Calibrated to two point 12.9 mS/cm and 1413 uS/cm and the probe was reading 36-37 psu range. Closer to what was run at CSUN.
Resolution: We got our salinity values down to ~38 when re-measuring but even at room temperature these are still our average values. I think this has to do with our calibration solution or our probe.. Samples from January TP1 with high salinity but was 50 mL falcon tube: POR-70. Salinity values at URI are 2 units higher b/c the probe was reading 2 units higher than it should be. Correct for this in our data but not CSUN’s.

Replace URI’s salinity values with average salinity of CSUN (36.164)
Replace with that run’s “correct” salinity value (under 37) for Run 1, 2, and 3. This doesn’t solve other timepoint’s issue though.

Issue: 50 mL empty falcon tube bottles once done are sitting in the completed bin.
Resolution: Figure out the waste protocol for these bottles since they had mercuric chloride in them. Wait until processing is done to get rid of them. More of a reminder than an active issue.

Issue: In script, where does the umol.cm2.hr calc come from? (deltaTA/2)(1.023)((vol.L1000)/surface.area.cm2)(1/timediff)(1/1000). And where does the 36 coefficient for normalizing TA come from?
*Resolution: Find the paper source and/or make a note of this in the script or in the paper.

Salinity issue

Are there any samples that were done at both URI and CSUN?

TA.norm = TA * Salinity/36

Initial 1 Run 3
CSUN:

TA: 2347.879
Salinity lab: 36.25
TA.norm: 2364.184

Putnam Lab:

TA: 2337.256
Salinity lab: 38.02
TA.norm: 2468.402

Are there any runs that have samples split between URI and CSUN?

Run 1 = 1 coral done at CSUN; the rest of the run done at URI Run 2 = all URI
Run 3 = 2 done at CSUN; the rest of the run done at URI

CSUN consistently below URI: ~36.35 psu and URI is ~37.5-38.5 psu.

Average for all CSUN samples = 36.164 psu

Options:

We could use average CSUN value for all samples.
Some URI samples are in the 36 range and I’d like to keep those.. So if any URI value is above a threshold (37.5?) we could replace that value with the average CSUN value or -1.5 psu from that value.
Would only work for September time point (bc the other 3 are only URI): Run 1 and 3 have CSUN values, we could replace URI salinity values with the CSUN salinity value for that run.

ALL TP:

effect of environment of chamber (Jan - none but no pH values, March - none, Sept- pH)
blanks and initial variation - some blanks have very low volume value (that isn’t realistic)
Differences in run #s
Salinity probe reading 2 units higher - correct for this in our data but not CSUN’s

January 2020 TP1:

Run 1-4 initials and blanks are wonky, same with Run 8 and 9
POC-248, POR-83 titration “NOT OK” from LabX output. Values seem to be OK though.
Environmental data: pH of chamber missing
2 blanks for Run 5 - 2 bottles run. I kept the first blank from deltaTA and that titration bottle (kept from 20200318 Run 2 not Run 1)
BK-2 lost water during volume measurement - 575 mL isn’t correct so I replaced 575 with 610 mL b/c chamber 1 was used again in later run for a blank so that is the empty volume for that chamber.
ACR 224 is bonus coral that doesn’t exist anywhere else

March 2020 TP2:

Only 1 initial for Run 11
No mismatches in data df
Runs 10-13 were much higher

September 2020 TP3:

Blank 3 and Blank 4 are both from chamber 10 but have different volumes – this shouldn’t be the case
The only missing samples: Initial1_20200909_1 and POR-240_20200913_5.
There are 2 Initial1_20200912_3 bottles/TA values (one bottle at URI but two TA values - one done at URI and one done in CA). Could one of these be for Run 4 on the same day?
Initial environmental measurements (temp, pH, salinity) missing run 3 and on.
Run 4 missing both both initial bottles.
Run 1-3 high blanks and initial values

November 2020 TP4:

Initial 2 from Run 2 taken out b/c way lower than expected

List for Danielle to bring back from Mo’orea Spring 2022

September 2020: Run 4 BK-4 : DANIELLE FOUND
September 2020: Run 4 Initial 1 and 2
September 2020: POR-240 Run 5 20200913 : DANIELLE FOUND

Closed Issues

Issue: We are currently copy and pasting into Timepoint#_TA_Data in urol timeseries repo.
Resolution: Emma created a new script for calc rates that pulls in raw files.

Issue: Do we use chamber temperature, salinity, and pH? Check with Hollie and Nyssa.
Resolution: In R script, we can look at TA as a function of these values to see if we need to correct for anything.

Issue: All September bottles have been processed but there are several blank and initials that we do not have accounted for. Cross reference again with those bottles done at CSUN and in-field notes.
Resolution: Ariana and Emma are currently chatting about the best way to go about this. Some bottles spilled in transport but we don’t think these are them. Turns out my code wasn’t pulling all the California bottles done. The only missing samples from September are and Initial1_20200909_1 and POR-240_20200913_5. There are 2 Initial1_20200912_3 bottles/TA values (one bottle at URI but two TA values - one done at URI and one done in CA).

Issue: Sample notation was not consistent for the first couple of rounds.
Resolution: Changed initial notation format on 0303 in the E5 timeseries folder and changed to all correct format on the titrator computer.

Issue: volumes missing from delta TA sheet.
Resolution: https://drive.google.com/drive/u/0/folders/1Z7Gxiqo8QbnP4AMpgxmgq0wDNJ-qWmBW. extract volumes here and put in delta TA.

Issue: Address variation in initials and blanks.
Resolution: Cross comparison between initial 1 and 2, across days to see variation, and the same for the blanks. More of a reminder to do than an active issue (unless there is larger variation than expected).

Issue: TP1 - January surface area is 30 for every chamber.
Resolution: pull in real surface area for this.

Issue: 50 mL falcon tubes for some samples for January 2020 and November 2020 time points instead of larger bottles.
Resolution: Hollie says it doesn’t matter we can run these at whatever mass we can get - run this by Ariana and Danielle in meeting. Will add a column to keep track of which samples were in a 50 mL and we can see if there is a batch effect. For these samples, we will need to calculate salinity prior to measuring mass and putting samples in rondolino.

Issue: 20220314 titration run - ACR-246 and Initial 2 may be mixed up in the rondolino (mass and salinities are correct, just position in titrator). Almost positive I fixed it but double check TA values at the end to make sure these make sense.
Resolution: Emma looked and samples were correct and fixed.

Weekly Schedule

Emma will sign the Putnam lab up for the appropriate time slots at the beginning of the week on the PPP team up calendar. Titrations google sheet schedule.

Week of 3/7 - 3/11

	Monday 3/7	Tuesday 3/8	Wednesday 3/9	Thursday 3/10	Friday 3/11
Personnel		Emma starts (~9 am); Kristen finishes (12:15-3:15p)	Kristen starts (~9a-1p); Emma finishes (whenever Run2 is done)	Emma starts (~9 am-12p); Megan ends (12:15p - end)	Amy
E5 sample runs	0	2	2	2	0
Notes			2 E5 runs; 1 blue tank run	Emma does pH cal, CRM run, 1 E5 round; Megan (Puritz lab) runs 1-2 sample runs	Puritz Lab

# of runs	Samples done this week
6	48

Week of 3/14 - 3/18

	Monday 3/14	Tuesday 3/15	Wednesday 3/16	Tuesday 3/1	Friday 3/18
Personnel	Emma and Pierrick together all day	Puritz Lab	No one - power out	Emma and Kristen	Pierrick, Emma, Hollie
E5 sample runs	3	0	0	4	3
Notes	Kristen out M-W for spring break

# of runs	Samples done this week
10	80

Week of 3/21 - 3/25

	Monday 3/21	Tuesday 3/22	Wednesday 3/23	Thursday 3/24	Friday 3/25
Personnel	Pierrick and Emma	Emma starts (~8 am); Kristen finishes (12:15-3:15p)	Kristen starts (~9a-1p); Emma finishes (whenever Run2 is done)	Puritz lab all day	Pierrick all day
E5 sample runs	3	4	3	0	2
Notes

# of runs	Samples done this week
12	96

Week of 3/28 - 4/1

	Monday 3/28	Tuesday 3/29	Wednesday 3/30	Thursday 3/31	Friday 4/1
Personnel		Kristen (12:15-3:15p)	Kristen (~9a-1p)	Kristen afternoon	Puritz lab all day
E5 sample runs	0	1	1	1	0
Notes

# of runs	Samples done this week
3	24

Week of 4/4 - 4/8

	Monday 4/4	Tuesday 4/5	Wednesday 4/6	Thursday 4/7	Friday 4/8
Personnel	Emma	Emma and Kristen	Emma	Emma	Puritz all day
E5 sample runs	3	3	4	3	0
Notes			Kristen out	Kristen out

Projected:

# of runs	Samples done this week
13	104

September 2020, 51 samples to complete

20220303 Kristen and Emma (1 run = 8 samples)

CRM error: -0.11796%

SampleID	TA	Mass	Salinity
JUNK 1	2535.93082	59.733	35
INITIAL1_20200912_3	2337.25626	60.379	40.34
INITIAL2_20200912_3	2348.79131	60.446	40.41
ACR-343_20200912_3	2333.80991	60.081	40.35
POR-340_20200912_3	2218.77808	59.626	40.16
POR-362_20200912_3	2265.81267	60.031	40.29
POR-381_20200912_3	2145.78802	59.934	40.3
POR-387_20200912_3	2295.43894	60.478	40.35
POR-373_20200912_3	2286.19554	60.173	40.26

20220308 Kristen and Emma (2 runs = 16 samples)

CRM error: -0.16%

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2538.00404	59.725	35
POR-338_20200909_2	2286.2164	60.178	40.61
Initial1_20200909_2	2331.52126	60.177	40.34
POR-383_20200909_2	2258.89333	60.472	40.18
POR-354_20200912_3	2267.02905	59.979	39.94
POR-353_20200909_1	2303.53562	59.46	39.94
POR-341_20200909_2	2221.63388	59.672	40.38
BK-2_20200909_2	2314.59019	59.823	39.87
POC-391_20200912_3	2319.23502	60.119	39.73

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2537.4245	60.207	35
Initial2_20200909_2	2349.06732	59.741	40.49
POC-359_20200909_1	2335.40506	59.578	40.52
POC-369_20200909_2	2286.28668	60.163	40.5
POC-378_20200909_2	2264.71636	59.926	40.63
POC-395_20200909_2	2315.40556	59.611	40.63
POR-349_20200909_2	2259.5831	60.468	40.66
POR-355_20200909_2	2269.82513	59.525	40.59
POR-384_20200909_2	2277.95517	60.238	40.7

20220309 Kristen and Emma (2 runs = 16 samples; 3 runs total for the day (1 blue tank run))

First E5 sample run was for September 2020 and the 2nd run was for January 2020 time point.

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2486.92729	60.388	35
BK1_20200909_1	2344.78601	60.492	40.7
Initial2_20200909_1	2346.26208	60.095	40.71
POC-358_20200909_1	2300.88123	60.436	40.74
POC-372_20200909_1	2299.23341	60.256	40.77
POC-386_20200909_1	2266.33998	60.454	40.84
POR-357_20200909_1	2293.5182	59.981	40.75
POC-365_20200909_1	2228.51312	60.359	40.84
POC-367_20200909_1	2246.40924	60.109	40.98

January 2020, 146 samples to complete

20220309 Kristen and Emma (2 runs = 16 samples; 3 runs total for the day (1 blue tank run))

First E5 sample run was for September 2020 and the 2nd run was for January 2020 time point.

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2483.8335	60.305	35
Initial2_20200107_8	2360.47591	59.997	40.96
POR-381_20200107_8	2306.57443	59.691	40.99
ACR-364_20200107_8	2341.16235	60.528	40.86
POC-373_20200107_8	2274.75422	60.144	40.72
POC-394_20200107_8	2349.66741	60.258	40.3
ACR-363_20200107_8	2326.21349	59.786	40.96
POR-357_20200107_8	2320.55603	60.547	40.81
Initial1_20200107_8	2354.24313	59.783	40.59

20220310 Emma (2 runs = 16 samples)

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2489.40784	58.13	35
POC-391_20200107_8	2323.87908	59.986	41.93
POC-254_20200104_1	2336.21906	60.298	41.14
Initial1_20200104_1	2355.49784	59.705	41.32
BK-8_20200107_8	2366.69776	60.216	41.8
Initial2_20200104_1	2350.25702	60.648	41.76
Initial2_20200108_9	2343.9948	59.581	41.58
Initial1_20200108_9	2308.83694	59.802	41.48
ACR-368_20200107_8	2330.2108	60.478	41.49

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2484.84902	59.981	35
ACR-267_20200104_2	2315.30616	59.511	41.47
ACR-228_20200104_2	2345.61169	60.255	41.27
POC-219_20200102_1	2321.50614	60.75	41.38
POR-214_20200104_1	2340.83917	59.614	40.93
ACR-256_20200104_2	2308.17129	59.637	41.42
POC-239_20200104_1	2332.69129	60.495	40.93
POR-242_20200104_1	2305.38159	59.97	40.31
POR-262_20200104_1	2204.22422	60.699	40.48

20200314 Emma and Pierrick (3 runs = 24 samples)

-0.13% CRM error

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2275.1106	59.205	35
Initial2_20200105_3	2286.70311	59.923	41.15
BK-3_20200105_3	2355.50141	60.611	42.33
POC-238_20200105_3	2333.09919	60.404	40.61
ACR-234_20200105_3	2272.03283	59.905	40.82
Initial1_20200104_2	2334.63197	59.795	40.12
ACR-229_20200105_3	2257.26957	59.706	41.24
POC-255_20200104_2	2292.36053	59.436	38.42
POC-201_20200104_2	2333.90785	59.729	41.52

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2274.50372	60.696	35
POC-205_20200105_4	2338.22994	59.888	41.54
ACR-246_20200105_4	2265.14352	60.031	41.17
POC-222_20200106_5	2319.27203	60.956	41.33
Initial2_20200105_4	2314.01441	59.993	41.62
Initial1_20200105_4	2356.00739	59.188	41.53
POC-200_20200105_3	2332.13855	60.718	41.42
POC-371_20200106_6	2329.36386	60.349	41.2
POR-209_20200105_3	2259.74708	60.426	40.63

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	2269.71589	59.584	35
POC-378_20200107_7	2300.71112	60.28	41.55
POR-383_20200107_7	2287.35238	60.604	41.3
POC-358_20200107_7	2320.07917	60.561	41.55
ACR-393_20200106_6	2333.50884	60.059	41.41
ACR-379_20200107_7	2330.53561	59.726	41.62
POR-260_20200106_5	2176.26568	60.658	41.55
POC-395_20200106_6	2308.54744	59.312	41.63
BK-4_20200105_4	2354.1675	59.679	41.82

20200317 Emma and Kristen (4 runs)

-0.06% error for CRMs

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2109.01085	58.53	35
Initial2_20200104_2	2323.45155	61.219	37.31
POR-221_20200104_1	2279.88571	60.32	36.8
POR-236_20200104_2	2304.69755	59.684	36.82
POR-235_20200104_2	2330.78335	60.356	37.65
ACR-247_20200104_1	2331.74976	61.125	37.4
BK-1_20200104_1	2356.03458	60.851	37.64
ACR-244_20200104_2	2312.64306	59.306	37.53
ACR-243_20200104_1	2313.96408	60.283	37.83

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2110.499	60.101	35
BK-6_20200106_6	2340.47914	60.051	37.87
POC-217_20200105_3	2328.82375	60.027	37.89
ACR-225_20200106_5	2295.83706	59.846	37.9
ACR-231_20200105_4	2302.29988	60.24	37.97
ACR-237_20200106_5	2301.59754	60.308	37.95
ACR-350_20200106_6	2324.14255	59.888	37.91
POR-240_20200105_4	2320.5666	60.421	37.97
POR-353_20200106_6	2248.87862	59.829	37.96

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	2108.20792	60.346	35
Initial1_20200106_6	2342.93012	60.118	37.87
Initial1_20200107_7	2359.76202	60.018	37.89
Initial2_20200106_6	2328.58517	59.684	37.91
Initial2_20200107_7	2358.01268	60.039	37.94
POR-340_20200107_7	2298.74556	59.68	37.98
POR-355_20200106_6	2292.79806	60.093	37.96
POR-384_20200107_7	2246.51929	60.055	37.96
ACR-343_20200107_7	2309.90034	60.242	38

Run 4:

SampleID	TA	Mass	Salinity
JUNK 1	2106.20673	60.064	35
Initial1_20200106_5	2289.58405	59.751	37.55
BK-7_20200107_7	2356.63365	59.634	37.54
POC-369_20200107_7	2322.27535	60.072	37.56
POC-377_20200106_6	2280.25736	59.803	37.65
ACR-265_20200105_4	2328.93355	60.273	37.7
ACR-345_20200107_7	2329.65971	60.455	37.89
POR-224_20200105_4	2320.6102	60.135	37.93
POR-362_20200106_6	2274.03636	60.256	37.94

20200318 Emma and Pierrick and Hollie (3 runs)

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2111.58105	60.763	35
POR-253_20200105_4	2200.01988	60.611	37.16
POR-251_20200106_5	2245.50642	60.02	37.14
POR-266_20200105_3	2241.96263	60.063	37.21
POC-207_20200105_4	2330.15529	59.83	37.17
POR-245_20200105_3	2297.85524	59.366	37.18
ACR-258_20200105_3	2329.42448	60.03	37.33
BK-5_20200106_5	2323.33425	60.164	37.24
POC-259_20200105_3	2274.74885	60.668	37.14

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2083.14922	59.95	35
Initial1_20200105_3	2273.21421	37.256	35.6
POR-261_20200106_5	2338.40199	59.738	37.61
BK-5_20200106_5	2356.92038	60.523	37.46
BK-2_20200104_2	2359.63364	60.817	37.51
ACR-389_20200106_6	2332.09833	59.824	38.77
POC-248_20200104_2	2125.46803	29.433	35.4
Initial2_20200106_5	2195.53421	27.034	36.49
Initial2_20200111_14	2341.65789	49.902	37.15

POC-216 bottle label is actually POR-261.

Run 3:

SampleID	TA	Mass	Salinity
POR-387_20200108_9	2305.25321	51.915	37.46
ACR-145_20200111_14	2328.40214	48.758	37.49
ACR-165_20200111_14	2292.71005	51.182	37.55
POC-55_20200111_14	2324.54047	48.309	37.62
POC-53_20200111_14	2331.14166	50.319	37.6
POR-73_20200111_14	2347.30681	49.319	37.22
POR-82_20200111_14	2290.74187	52.039	37.24
POC-57_20200111_14	2397.20882	43.858	38.71
POC-372_20200108_9	2318.10586	51.839	37.24

20200321 Emma and Pierrick (3 runs)

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2118.57179	45.928	35
POC-45_20200111_15	2331.34309	49.453	37.63
POR-74_20200111_14	2226.30763	49.906	37.42
BK-14_20200111_14	2341.3981	51.681	36.79
Initial1_20200111_14	2337.796	50.312	37.71
ACR-185_20200111_14	2287.78789	51.132	36.84
POC-50_20200111_15	2325.35604	50.158	36.24
POC-68_20200111_15	2323.62506	49.396	36.2
POR-83_20200111_15	2242.81069	50.659	37.42

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2642.5321	60.967	35
POR-77_20200111_15	2320.2919	51.663	37.72
ACR-175_20200111_15	2326.74355	48.851	37.93
ACR-186_20200111_15	2321.95558	50.424	37.75
BK-15_20200111_15	2373.52763	44.484	37.71
POC-50_20200111_15	2326.6607	49.114	37.5
ACR-173_20200111_15	2340.83327	45.174	38.47
POR-72_20200111_15	2262.39016	49.13	38.06
Initial1_20200111_15	2343.90542	50.253	37.78

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	2646.21995	59.184	35
NA	2333.77641	47.405	37.66
NA	2244.98889	50.501	37.95
NA	2328.34115	50.149	38.06
NA	2326.72068	48.348	38.74
NA	2314.87429	50.193	37.99
Initial2_20200111_15	2347.33022	51.522	37.89
NA	2325.83054	49.661	38.07
NA	2263.24728	48.634	38.21

See below note for NAs.

Duplicates done. These are taken out of the data files in the E5 folder for now but raw data lives in Titrator repo (not edited):

ACR-186 taken out of Run 3, kept in Run 2
POC-68 taken out of Run 3, kept in Run 1
POC-45 taken out of Run 3, kept in Run 1
ACR-173 taken out of Run 3, kept in Run 2
POR-83 taken out of Run 3, kept in Run 1
POR-77 taken out of Run 3, kept in Run 2
POR-72 taken out of Run 3, kept in Run 1

20200322 Emma and Kristen (4 runs)

CRM error: -0.05%

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2650.48042	44.108	35
ACR-139_20200110_13	2319.51332	50.256	37.17
POR-81_20200110_13	2314.74675	50.735	37.34
ACR-187_20200110_13	2292.28379	51.731	37.3
BK-13_20200110_13	2342.83092	50.645	37.47
POR-79_20200110_13	2258.19395	51.79	37.07
POR-71_20200110_13	2314.3771	50.08	37.9
POC-52_20200110_13	2310.9354	49.71	37.44
ACR-190_20200110_13	2316.44093	50.497	37.64

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2647.59596	55.731	35
ACR-176_20200110_12	2243.91882	51.99	36.98
POC-56_20200110_12	2323.0274	51.709	37.21
POR-69_20200110_12	2280.55526	53.379	37.18
POR-75_20200110_12	2246.50921	49.872	37.2
Initial2_20200110_13	2370.49297	47.291	37.73
Initial2_20200110_12	2337.9261	49.897	37.47
POC-41_20200110_13	2306.66837	49.607	37.28
POC-43_20200110_13	2309.73417	50.101	37.4

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	2645.13668	59.69	35
ACR-51_20200109_11	2323.50066	49.59	37.84
ACR-140_20200110_12	2295.45644	50.685	37.73
ACR-180_20200109_11	2323.09432	50.718	38.2
ACR-193_20200110_12	2288.35923	51.965	37.87
POC-47_20200110_12	2293.71726	52.933	38.18
POC-48_20200110_12	2292.89801	51.152	38.12
BK-12_20200110_12	2332.94472	51.275	38.06
POR-78_20200110_12	2245.37962	52.171	38.14

Run 4:

SampleID	TA	Mass	Salinity
JUNK 1	2643.79377	59.707	35
POR-70_20200109_11	2342.65017	47.102	41.18
POR-76_20200109_11	2314.69552	51.649	37.55
POR-80_20200109_11	2339.53291	48.432	37.83
ACR-150_20200109_11	2293.27016	46.331	37.93
ACR-396_20200108_9	2298.18117	54.322	37.22
BK-11_20200109_11	2338.51884	49.935	37.6
POC-42_20200109_11	2319.72446	48.979	37.82
POC-44_20200109_11	2387.20571	48.055	38.71

20200323 Emma and Kristen (3 E5 runs; 4 runs total with 1 blue tank run)

0.13% CRM error

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2994.82376	60.459	35
Initial2_20200109_11	2347.68385	50.295	37.23
POC-40_20200109_11	2336.42257	48.12	37.75
POC-346_20200108_9	2311.97058	45.69	37.33
POC-366_20200108_9	2305.23846	51.215	37.3
ACR-390_20200108_9	2313.38028	52.575	37.38
POR-338_20200108_10	2264.61331	50.293	37.65
POR-349_20200108_10	2313.13904	50.982	37.91
POR-354_20200108_9	2289.3542	51.392	37.96

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2973.47483	59.946	35
Initial2_20200108_10	2342.27335	51.074	37.41
POR-365_20200108_10	2317.11089	50.306	37.66
POR-367_20200108_10	2324.75939	50.397	37.55
BK-10_20200108_10	2345.53728	50.996	38.03
ACR-351_20200108_10	2306.23493	48.197	37.32
ACR-374_20200108_10	2308.43032	50.484	37.65
POC-375_20200108_10	2320.45236	50.271	37.57
POC-386_20200108_10	2343.08793	49.01	37.94

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	3002.88929	44.216	35
Initial1_20200110_12	2343.18073	53.353	37.01
Initial1_20200110_13	2344.21063	51.878	37.27
Initial1_20200108_10	2347.6633	49.497	37.25
Initial1_20200109_11	2337.01936	49.022	37.28
BK-9_20200108_9	2343.82123	50.195	37.64
POC-359_20200108_9	2329.67509	50.059	37.62
POR-341_20200107_8	2334.40555	56.833	38.21

November 2020, 145 samples to complete

20220325

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2459.52806	59.85	35
Initial2_20201103_3	2328.08088	59.597	38.46
Initial1_20201102_2	2344.52274	59.003	38.38
Initial1_20201102_1	2283.39532	60.133	38.65
BK-3_20201103_3	2327.75499	59.192	38.46
POC-248_20201103_3	2284.3558	60.599	38.38
Initial1_20201105_8	2347.90306	60.791	38.5
POR-209_20201102_1	2285.07298	59.812	38.64
ACR-241_20201103_4	2305.81769	59.832	38.51

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2459.19918	59.523	35
ACR-398_20201104_6	2330.44006	60.171	38.15
POR-242_20201103_3	2308.52711	59.267	38.19
POC-371_20201104_6	2322.18042	60.5	38.26
POC-255_20201103_4	2333.55238	60.396	38.44
POC-373_20201104_6	2312.65502	60.412	38.46
ACR-267_20201103_4	2207.2942	59.97	38.47
POC-207_20201102_1	2324.88341	60.167	38.34
BK-1_20201102_1	2348.63579	60.244	38.44

20220329

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2470.81103	59.913	35
Initial1_20201105_7	2375.6211	59.944	38.74
POR-362_20201105_7	2241.1036	59.764	38.51
POC-386_20201105_7	2316.37118	59.829	38.5
ACR-244_20201102_1	2278.36467	60.471	38.31
POR-383_20201105_8	2317.13254	59.813	38.47
ACR-237_20201102_1	2158.40301	60.309	38.53
ACR-229_20201103_3	2288.22038	60.121	38.51
Initial1_20201103_4	2350.70154	59.984	38.65

20220330

Run1:

SampleID	TA	Mass	Salinity
JUNK 1	1556.04272	59.872	35
POR_224_20201102_2	1982.24103	59.795	38.53
Initial2_20201102_2	2070.523	60.014	38.55
ACR-225_20201103_3	2272.05144	59.752	38.64
POR-221_20201103_3	2233.26146	60.259	38.76
POC-259_20201103_4	2135.60169	60.289	38.67
ACR-389_20201104_6	2340.14637	59.756	38.73
POC-372_20201104_6	2226.12092	60.127	38.66
POC-366_20201104_5	2042.30818	60.216	38.73

20220331

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2685.99576	59.669	35
Initial1_20201103_3	2357.93901	59.627	38.53
POC-238_20201102_2	2257.75923	60.284	38.64
POR-260_20201102_2	2274.71246	60.457	38.72
POR-214_20201102_1	2200.7441	59.989	38.69
ACR-210_20201102_2	2286.12298	60.5	38.62
POC-219_20201102_2	2271.37031	60.178	38.71
POR-216_20201102_1	2277.59854	59.841	38.71
ACR-213_20201102_2	2296.61666	59.943	38.79

20220404

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2215.25079	42.063	35
POC-346_20201105_8	2296.8305	51.609	39.03
POR-365_20201104_6	2260.39111	54.976	39.29
POR-340_20201104_5	2279.67102	59.552	39.18
POC-358_20201104_5	2328.00067	57.809	39.28
ACR-220_20201103_3	2253.65176	57.133	39.19
POC-369_20201104_5	2332.31998	59.956	39.26
POC-254_20201103_3	2302.67953	58.129	39.32
ACR-343_20201104_5	2156.2942	57.035	39.3

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2449.07902	53.995	35
ACR-256_20201103_4	2324.42984	54.088	39.19
Initial2_20201104_6	2351.01021	56.516	39.21
POC-359_20201104_5	2299.02915	53.227	39.43
POR-349_20201104_6	2313.73717	55.566	39.17
POR-341_20201104_5	2227.52434	53.448	39.33
POR-353_20201104_6	2189.43197	59.455	39.26
BK-6_20201104_6	2312.03214	56.003	39.29
ACR-368_20201104_5	2279.81497	57.319	39.35

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	2446.89246	59.876	35
POR-251_20201103_4	2293.29224	59.387	39.29
Initial1_20201104_6	2342.07398	59.896	39.22
POR-262_20201103_4	2266.24457	60.434	39.26
Initial1_20201104_5	2343.47258	60.971	39.27
POR-338_20201104_5	2288.88806	60.698	39.16
POR-245_20201103_3	2261.05437	60.193	39.18
POR-354_20201104_6	2296.80522	59.555	39.08
BK-5_20201104_5	2347.686	60.532	39.23

20220405

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2455.19823	55.633	35
Initial2_20201106_9	2319.0847	52.456	38.24
Initial2_20201106_10	2302.00178	52.931	38.14
POR-73_20201106_10	2264.46342	52.683	38.64
POC-40_20201106_9	2278.31715	53.372	38.11
ACR-145_20201106_9	2277.65617	54.957	38.2
POR-70_20201106_9	2575.80824	46.026	45.87
POR-74_20201106_10	2173.01456	52.571	38.24
BK-10_20201106_10	2312.12355	53.495	38.5

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2459.29679	59.988	35
POC-47_20201106_10	2375.5343	51.096	41.06
POR-72_20201106_10	2243.16768	52.797	39.62
ACR-175_20201106_10	2299.00502	53.497	39.09
POC-44_20201106_10	2312.11909	53.392	39.45
POC-43_20201106_9	2279.50746	53.086	39.33
POR-69_20201106_9	2214.73618	55.09	39.22
POR-71_20201106_9	2294.02013	52.441	40.5
Initial1_20201106_10	2313.52694	52.299	39.07

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	2454.84057	59.813	35
ACR-150_20201106_9	2390.2876	51.01	40.86
BK-8_20201105_8	2312.99331	53.103	39.46
ACR-176_20201106_10	2273.41324	53.903	39.22
Initial1_20201106_9	2312.1421	51.567	39.18
ACR-173_20201106_10	2293.94975	54.57	39.2
POC-45_20201106_10	2281.9282	53.781	39.3
ACR-139_20201106_9	2406.96171	51.684	41.24
BK-9_20201106_9	2309.69362	53.303	39.21

20220406

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2453.36554	51.4	35
POR-357_20201105_7	2287.31341	56.134	39.5
POC-391_20201105_8	2315.54577	56.953	39.25
POC-375_20201105_7	2318.89853	58.838	38.89
Initial2_20201105_7	2345.46256	54.44	39.03
POC-394_20201105_8	2314.7015	55.353	38.91
POC-377_20201105_7	2317.55119	54.729	38.91
POC-378_20201105_7	2247.99033	56.844	38.71
ACR-234_20201102_1	2233.43928	54.75	38.98

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2454.79681	53.023	35
POC-42_20201106_9	2279.72266	52.572	39.39
POR-240_20201102_2	2304.73126	55.106	39.54
Initial2_20201105_8	2340.89755	57.739	39.49
Initial2_20201102_1	2303.27971	61	39.47
ACR-390_20201105_7	2307.28893	59.167	39.46
BK-2_20201102_2	2255.41764	57.563	39.25
POR-384_20201105_8	2290.54093	56.42	39.28
POC-395_20201105_8	2324.36352	56.254	39.17

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	4095.35382	24.748	35
ACR-218_20201102_2	2194.16801	55.877	39.66
POC-205_20201102_1	2280.55671	54.129	39.67
POC-222_20201102_2	2314.33751	56.28	39.5
ACR-393_20201105_8	2328.77103	53.989	39.65
POR-367_20201105_7	2268.30316	59.917	39.2
POC-200_20201102_1	2309.16733	56.301	39.63
POR-381_20201105_8	2190.69777	60.546	39.37
BK-7_20201105_7	2342.26238	57.006	39.43

Run 4:

SampleID	TA	Mass	Salinity
POC-50_20201107_12	2295.59409	53.408	39.7
POR-385_20201105_8	2255.15972	52.336	39.59
POC-239_20201103_3	2325.1578	54.049	39.49
Initial2_20201103_4	2343.93203	56.65	39.43
POC-257_20201103_4	2321.67537	55.956	39.6
BK-4_20201103_4	2343.80303	57.351	39.6
POR-253_20201103_4	2292.41839	56.323	39.48
Initial2_20201104_5	2346.28827	59.052	39.54

20220407

Run 1:

SampleID	TA	Mass	Salinity
JUNK 1	2462.74103	48.867	35
POR-75_20201107_11	2266.84129	51.645	39.16
ACR-178_20201107_11	2333.26496	52.936	39.9
POC-52_20201107_11	2328.66141	52.31	39.14
POC-48_20201107_12	2310.66864	53.033	38.9
ACR-186_20201107_12	2287.20802	54.479	38.21
POC-55_20201107_11	2298.00034	54.821	37.83
ACR-185_20201107_11	2403.8172	52.244	39.46
ACR-180_20201107_11	2308.76516	54.219	37.99

Run 2:

SampleID	TA	Mass	Salinity
JUNK 1	2466.76751	48.956	35
POC-68_20201107_12	2298.86364	53.24	37.61
POC-53_20201107_11	2292.89003	54.839	39.06
BK-11_20201107_11	2383.68147	53.059	39.73
POC-57_20201107_12	2401.55067	51.579	39.6
ACR-187_20201107_12	2285.52314	54.9	37.42
POR-79_20201107_11	2237.75686	54.288	37.35
POR-76_20201107_11	2161.95406	54.583	37.01
Initial1_20201107_12	2322.32984	53.464	38.72

Run 3:

SampleID	TA	Mass	Salinity
JUNK 1	2459.95448	58.154	35
BK-12_20201107_12	2315.17967	54.23	38.59
Initial2_20201107_11	2323.62604	52.308	38.47
Initial1_20201107_11	2339.57832	51.364	39.3
POR-82_20201107_12	2204.67128	54.183	38.1
POR-81_20201107_12	2546.20421	46.797	42.17
POR-83_20201107_12	2158.60913	54.008	37.96
Initial2_20201107_12	2313.85678	51.491	38.7

Salinity probe test info

Equipment locations

Lab inventory link google sheet: here.

More 10 mL serological pipettes live on BS11.

BS10: titrator supplies, buffers, pH
BS11: ziplock bags and plastic containers, 10 mL serological pipette tips

Junk seawater can be taken from a blue tank downstairs in the aquarium room.

The serological pipette holder lives in the third drawer down on the same bench as the waste (just the left side if you are looking at the bench).

Waste

See titration protocol for full waste details.

Serological pipette tips are broken in half and placed in the solids bin. Kim wipes are also placed in this bin.
Samples post-run are placed in the liquids container.

Waste pick up

When the bottles are ready to be picked up, fill out this link.

From: Emma Strand
Email Address: emma_strand@uri.edu
Location of Waste: CBLS 190
PI: Hollie Putnam
Waste Container 1-List all contents in waste container: Insert all contents on the bottle label.
Quantity and size of container: 4L plastic jug
Hazard Class: toxic
Repeat for as many bottles as we have.

Supplies Needed (Use dropdown menu below description to indicate quantity needed):

4 liter plastic jug (for liquid wastes): 6

Additional comments: The bottles are located in the first row of benches in CBLS 190. Bottles to be picked up are marked with tape and “To Be Picked Up” label in cabinet labeled BD12.

Acid Bottle replacement

Purge the remaining acid out so that the lines and bottle are empty.
This acid is now waste. This can be neutralized with seawater or DI water and poured down the sink (contact PI to make sure you are doing this step correctly).
Connect the new acid bottle to the titrator lines and purge at least six times to fill the lines with the new acid.

Written on March 3, 2022